Journal: Oncology Reports

Article Title: miR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma

doi: 10.3892/or.2020.7601

Figure Lengend Snippet: Oligonucleotides sequences used for transfection.

Article Snippet: Synthetic miR-193b mimic, miR-193b inhibitor, negative controls (NC and inhibitor NC), silencer Select Negative Control (siNC), and KRAS, STMN1 and MYC small interfering RNAs (siRNAs) were obtained from Shanghai GenePharma Co., Ltd. Lentiviral particles containing miR-193b (LV-193b), miR-193b inhibitor (LV-193b inhibitor) and negative controls (LV-NC and LV-inhibitor-NC) were purchased from GeneCopoeia, Inc.

Techniques: Transfection, Sequencing

Journal: Journal of Breast Cancer

Article Title: miR-195/miR-497 Regulate CD274 Expression of Immune Regulatory Ligands in Triple-Negative Breast Cancer

doi: 10.4048/jbc.2018.21.e60

Figure Lengend Snippet: CD80 =cluster of differentiation 80; CD86 =cluster of differentiation 86; CD274 =cluster of differentiation 274; ICOSLG =inducible T-cell costimulator ligand; CD276 =cluster of differentiation 276; VTCN1 =V-set domain containing T cell activation inhibitor 1; C10orf54 =chromosome 10 open reading frame 54; NCR3LG1 =natural killer cell cytotoxicity receptor 3 ligand 1; HHLA2 =HERV-H LTR-associating 2; PDCD1LG2 =programmed cell death 1 ligand 2. * p <0.05.

Article Snippet: The wild-type CD274 3′UTR was cloned into the pmiR-RB-REPORTTM vector (RiboBio).

Techniques: Activation Assay

Journal: Journal of Breast Cancer

Article Title: miR-195/miR-497 Regulate CD274 Expression of Immune Regulatory Ligands in Triple-Negative Breast Cancer

doi: 10.4048/jbc.2018.21.e60

Figure Lengend Snippet: CD274 =cluster of differentiation 274; HHLA2 =HERV-H LTR-associating 2; PDCD1LG2 =programmed cell death 1 ligand 2; CD276 =cluster of differentiation 276; ICOSLG =inducible T-cell costimulator ligand; NCR3LG1 =natural killer cell cytotoxicity receptor 3 ligand 1. * p <0.05.

Article Snippet: The wild-type CD274 3′UTR was cloned into the pmiR-RB-REPORTTM vector (RiboBio).

Techniques:

Journal: Journal of Breast Cancer

Article Title: miR-195/miR-497 Regulate CD274 Expression of Immune Regulatory Ligands in Triple-Negative Breast Cancer

doi: 10.4048/jbc.2018.21.e60

Figure Lengend Snippet: CD80 =cluster of differentiation 80; CD86 =cluster of differentiation 86; CD274 =cluster of differentiation 274; ICOSLG =inducible T-cell costimulator ligand; CD276 =cluster of differentiation 276; VTCN1 =V-set domain containing T cell activation inhibitor 1; C10orf54 =chromosome 10 open reading frame 54; NCR3LG1 =natural killer cell cytotoxicity receptor 3 ligand 1; HHLA2 =HERV-H LTR-associating 2; PDCD1LG2 =programmed cell death 1 ligand 2.

Article Snippet: The wild-type CD274 3′UTR was cloned into the pmiR-RB-REPORTTM vector (RiboBio).

Techniques: Activation Assay

Journal: Journal of Breast Cancer

Article Title: miR-195/miR-497 Regulate CD274 Expression of Immune Regulatory Ligands in Triple-Negative Breast Cancer

doi: 10.4048/jbc.2018.21.e60

Figure Lengend Snippet: CD274 =cluster of differentiation; PD-L1=programmed death-ligand 1. * p <0.05; † p <0.01.

Article Snippet: The wild-type CD274 3′UTR was cloned into the pmiR-RB-REPORTTM vector (RiboBio).

Techniques:

Journal: Oncotarget

Article Title: Positive regulation of TAZ expression by EBV-LMP1 contributes to cell proliferation and epithelial-mesenchymal transition in nasopharyngeal carcinoma

doi: 10.18632/oncotarget.13775

Figure Lengend Snippet: A . Induction of F-actin rearrangement by LMP1. CNE1-EV, CNE1-LMP1 and CNE1-LMP1-ΔCT (deletion of the C-terminus) cells were grown on poly-L-lysine-coated coverslips overnight. Cells were fixed, and stained with a FITC-conjugated phalloidin (Green). The expression of LMP1 led to formation of the microspike-like actin structures (filopodia) at the plasma membrane (arrows). Images were acquired using a Leica microscope as detailed in Materials and Methods. Scale bars, 50 mm. Transfection of an empty vector had no effect on actin organization. B and C . LMP1 or LMP1-ΔCT interacts with gelsolin. Lysates from HEK 293T cells transfected with the indicated combinations of WT or truncation mutants of Flag-tagged LMP1 were subjected to anti-Flag IP followed by anti-Flag and anti-gelsolin IB. D . Effect of gelsolin on LMP1-induced TAZ signaling. CNE1-EV/CNE1-LMP1 cells were analyzed by western blot after transfection with gelsolin siRNA using antibodies to p-Lats1/2, Lats1/2, TAZ and tubulin. E . Proposed mechanism for LMP1 promotion of TAZ stability.

Article Snippet: siRNAs against gelsolin and scrambled control were synthesized by Genepharma (Suzhou, China).

Techniques: Staining, Expressing, Clinical Proteomics, Membrane, Microscopy, Transfection, Plasmid Preparation, Western Blot

Journal: Oncotarget

Article Title: Positive regulation of TAZ expression by EBV-LMP1 contributes to cell proliferation and epithelial-mesenchymal transition in nasopharyngeal carcinoma

doi: 10.18632/oncotarget.13775

Figure Lengend Snippet: LMP1 interaction with gelsolin contributes to actin cytoskeleton remodeling, and thus inhibits Lats1/2 phosphorylation and enhances TAZ stability, causing cell proliferation, EMT and stemness.

Article Snippet: siRNAs against gelsolin and scrambled control were synthesized by Genepharma (Suzhou, China).

Techniques: Phospho-proteomics

Journal: Nature Communications

Article Title: Ferroptosis spreads to neighboring cells via plasma membrane contacts

doi: 10.1038/s41467-025-58175-w

Figure Lengend Snippet: Opto-GPX4Deg and bystander HeLa ( a – d ), SCLC ( e – h ), or HT29 ( i – l ) cells transfected with either siRNA against α-catenin or scramble siRNA (control), and treated or not with 5 µM Fer-1. a , e , i Kinetics of cell death in α-catenin KD and control samples. b , f , j %AUC for indicated populations. Experiments were performed with three independent biological replicates ( n = 3). c , g , k WB analysis of indicated protein levels in α-catenin KD and control cells. d , h , l Quantification of WB in ( c , g , k ). Protein levels normalized to loading control. Experiments were performed with three independent biological replicates ( n = 3). m Kinetics of cell death in Opto-GPX4Deg and bystander HeLa cells transfected with RFP-tagged empty vector, WT E-cadherin, E-cadherin ΔCBD mutant, WT N-cadherin or WT P-cadherin. n %AUC for the cell populations in ( m ) Experiments were performed with three independent biological replicates ( n = 3). Statistical analysis by two-sided one-way ANOVA corrected for multiple comparisons using Tukey’s multiple comparison test or by parametric t -test ( d , h , l ). Exact p values are shown. All experiments were performed with three independent biological replicates ( n = 3). Values are displayed as mean ± SD.

Article Snippet: For every well, 20 pmol of α-catenin siRNA (GGAGCCAGCUAGAUAUUAA, SiTools Biotech) or siRNA control/non-targeting (D-001810-0120, SiTools Biotech) was premixed in 100 μl Opti-MEM (Thermo Fisher) and 1:1 mixed with 5-μl Lipofectamine RNAiMAX (Invitrogen) or PEI (see cell transfections) diluted in 100 μl Opti-MEM.

Techniques: Transfection, Control, Plasmid Preparation, Mutagenesis, Comparison

Journal: Experimental and Therapeutic Medicine

Article Title: miRNA-30d serves a critical function in colorectal cancer initiation, progression and invasion via directly targeting the GNA13 gene

doi: 10.3892/etm.2018.6902

Figure Lengend Snippet: Tumor suppressive effects of miR-30d via GNA13 expression inhibition. (A and B) Expression of (A) miR-30d expression and (B) GNA13 was evaluated by RT-qPCR and western blot analysis, respectively, in SW480 cells co-transfected with miR-30d mimic (or NC mimic) and GNA13 [or pcDNA3.1(+)]. *P<0.05 GNA13 vs. pcDNA3. (C-E) Transwell migration and invasion assays. (C) Representative images (magnification, ×200) of Transwell assays; (D and E) quantitative analysis of migration and invasion. *P<0.05, **P<0.01. (F) SW480 cells were transfected with siGNA13 or siNC. GNA13 expression was determined by western blotting after 48 h. (G and H) Following transfection with siRNA or siNC, Transwell migration and invasion assays were performed. (G) Representative images (magnification, ×200) of Transwell assays; (H) quantitative analysis of migration and invasion. *P<0.05, **P<0.01 vs. siNC. miR, microRNA; NC, negative control; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; siRNA or si, small interfering RNA.

Article Snippet: SiRNA for GNA13 (cat. no. MBS8207766) and siRNA negative control (cat. no. MBS8241404) were obtained from MyBioSource, Inc. (San Diego, CA, USA).

Techniques: Expressing, Inhibition, Quantitative RT-PCR, Western Blot, Transfection, Migration, Negative Control, Real-time Polymerase Chain Reaction, Small Interfering RNA